FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l05n01 ah8440a-062415.3410000000d596.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l05n01 ah8440a-062415.3410000000d596.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1897694
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG34365118.1088731903036TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATT22570.11893382178580951TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGGATG22330.11766912895335076TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA399200.044.9181371
TCGGAAG405550.044.1294443
GCACACG399050.044.1279611
ATCGGAA406800.044.060212
CACACGT400800.044.01949712
ACACGTC401000.043.99754313
GAGCACA402950.043.992189
AGAGCAC403550.043.982538
AGCACAC401300.043.9702610
CACGTCT400550.043.9323114
CGGAAGA407650.043.907634
ACGTCTG402000.043.86898815
CGTCTGA402000.043.80182616
GAAGAGC407500.043.7526476
GTCTGAA404550.043.6879817
GGAAGAG410150.043.6290325
TCGTATG393400.043.60751345
ACTCCAG400050.043.57205223
GAACTCC401400.043.5712321
TCTGAAC403550.043.56209618
Produced by FastQC (version 0.11.2)