FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l05n01 ah8383b-062415.3410000000d8e3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l05n01 ah8383b-062415.3410000000d8e3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343708
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC29770.8661421904639985TruSeq Adapter, Index 7 (100% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA15340.44630907630895994No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC12000.34913356686489694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCCG2000.041.6252716
CTCGGGG1800.041.25026716
CACACGT2900.040.3450912
TCGGGGA2250.040.0002617
CACGTCT3000.039.75025614
ATCCCTG2050.039.5124536
GTCTCCC2300.039.13068842
GGCGGTC2300.039.13068838
ACACGTC3000.039.0002513
AGTCACC3000.039.0002528
GCACACG3000.039.0002511
GATCGGA3150.038.639141
CTCCAGT3100.038.46239524
ACGTCTG3050.038.36090515
CATCTCG2950.038.14138839
CCTCGGG1950.038.07716815
AGCCGAT2200.037.8356518
TCGGAAG3300.037.494793
TCCCATC2500.036.90023845
CGGTCTC2450.036.74027640
Produced by FastQC (version 0.11.2)