FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l05n01 ah8115b-062415.3410000000d737.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l05n01 ah8115b-062415.3410000000d737.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences758290
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG171482.2614039483574886TruSeq Adapter, Index 18 (97% over 40bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA66840.8814569623758719No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC54840.7232061612311913No Hit
TCTTCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC13310.17552651360297514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCGGG5400.043.74288615
GCACACG20100.042.9780811
CACACGT20200.042.6539512
ACACGTC20000.042.63056613
AGCACAC20400.042.5666110
CACGTCT20100.042.53039614
AGTCACG19800.042.50710328
ACGTCTG20300.042.3330115
ACGTCCG19800.042.16613832
GAACTCC20400.042.12550421
GAGCACA20650.042.051279
ATCGGAA21150.042.0144422
CGTCCGC19900.041.838433
TCACGTC20100.041.76070830
CCCAGGC6850.041.71954334
TCGGAAG21250.041.710863
GTCACGT20200.041.66537529
CCAGTCA20350.041.4688526
GATCGGA21400.041.4677161
GTCTGAA20800.041.42354617