FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l05n01 ah8115a-062415.3410000000d6be.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l05n01 ah8115a-062415.3410000000d6be.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2904922
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC206500.7108624603345632TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG27600.038.8826411
ACACGTC28100.038.0306513
GATCGGA28600.037.5800321
CGTCTGA28350.037.53850616
GAGCACA28550.037.4305739
CACGTCT28500.037.33900514
GTATGCC27850.037.16476445
ACTGACC28250.037.1138832
CACACGT29050.037.01930212
ACGTCTG28900.036.90004715
AGCACAC29100.036.80043410
TCCAGTC29300.036.09039725
TCGGAAG30150.035.817923
CCAGTCA29800.035.63646726
GAACTCC30000.035.1732921
ATCTCGT29800.035.11279340
AGAGCAC30500.035.0380748
CTGACCA29950.035.00724833
CGTATGC29700.034.7746344
AGTCACT30350.034.76826528
Produced by FastQC (version 0.11.2)