FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l05n01 ah8104b-062415.3410000000d81c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l05n01 ah8104b-062415.3410000000d81c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences557104
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG6167411.070464401619805TruSeq Adapter, Index 19 (97% over 40bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA208643.7450817082627297No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC161372.8965866337344557No Hit
TCTTCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC37920.6806628564864011No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG67600.044.53029311
TCGGAAG68600.044.4714973
GATCGGA68850.044.4224321
AGCACAC68050.044.4011310
ACACGTC67850.044.36621513
CACACGT67800.044.3657512
AGAGCAC68600.044.340318
ACGTCTG67900.044.16787315
GAGCACA68750.044.1453939
ATCGGAA69150.044.117782
GTCTGAA68500.044.04375517
CACGTCT68250.044.04026814
TCTGAAC68200.044.03956218
CGTCTGA68100.044.0381616
CAGGGAT24900.043.9119836
AGGGATT24850.043.9098057
CGGAAGA69600.043.8971864
GAACTCC68150.043.84078621
ACTCCAG68250.043.8095223
AGTCACG68500.043.75208728
Produced by FastQC (version 0.11.2)