FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l05n01 ah8104a-062415.3410000000d7bd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l05n01 ah8104a-062415.3410000000d7bd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2783244
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC1459015.2421203458985275TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA161000.044.2667581
GCACACG160700.043.7232511
ACACGTC160650.043.6808413
CACACGT161200.043.57367312
GAGCACA162550.043.527799
CACGTCT161050.043.502514
ACGTCTG162000.043.3446115
TCGGAAG164450.043.3258553
CGTCTGA162250.043.28015516
AGCACAC162600.043.2384610
AGAGCAC163950.043.2109838
GAACTCC162500.043.00433321
TATCTCG161400.042.9961539
TCCAGTC161600.042.97776425
CCGATGT161350.042.97463633
ATCGGAA166150.042.9367182
GTATGCC161550.042.93068745
CCAGTCA162200.042.8341926
CTCGTAT162250.042.80325342
CAGTCAC162450.042.76750627