FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l04n01 ah8440b-062415.3410000000e27c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l04n01 ah8440b-062415.3410000000e27c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1930465
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC952074.931816945658171TruSeq Adapter, Index 2 (100% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA599133.1035527709645088No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC478912.4808012577280603No Hit
TCTTCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC110180.5707433183196794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG99500.043.8641711
TCGGAAG100150.043.8277173
CACACGT99800.043.73231512
ACACGTC99650.043.73041513
AGCACAC100350.043.55988310
GAGCACA100400.043.5393149
GATCGGA101450.043.3489071
ACGTCTG100600.043.31745515
ATCGGAA101650.043.3126452
AGAGCAC101150.043.260978
CGTCTGA100500.043.2486316
CACGTCT100700.043.20741314
TATCTCG98700.043.20070639
CTGACCC66450.043.07940340
GTCTGAA101100.042.9697117
CGATGTA100400.042.9578134
CCTCGGG54250.042.92120415
CCGATGT100550.042.8926233
GAACTCC101150.042.887321
TCGGGGA67000.042.8121117