FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l04n01 ah8383a-062415.3410000000e2a3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l04n01 ah8383a-062415.3410000000e2a3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432539
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC6008213.890539350208883TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA66250.044.5332681
GCACACG65800.044.4813711
AGAGCAC66100.044.3815888
ACACGTC66100.044.34755313
AGCACAC66200.044.31455210
GAGCACA66200.044.3145529
CACGTCT66300.044.28164314
CACACGT66250.044.24714712
ACGTCTG66200.044.24658215
TCGGAAG66850.044.0855873
GAACTCC65900.044.04853421
CGTCTGA66500.043.9454816
GTCTGAA66450.043.9108417
GTATGCC66250.043.8888345
ATCGGAA67300.043.8576662
CTGAACT66450.043.8431319
CGGAAGA67350.043.8251044
TCTGAAC66550.043.8110518
GAAGAGC67150.043.788126
CAGTCAC66750.043.68483427
Produced by FastQC (version 0.11.2)