FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l04n01 ah8335b-062415.3410000000e289.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l04n01 ah8335b-062415.3410000000e289.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1782173
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA62220.34912435549186305No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG57350.32179816437573683TruSeq Adapter, Index 18 (97% over 40bp)
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC53170.29834365126169005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCGGG6400.038.67071515
CTCGGGG6600.037.4988816
TCGGGGA8700.037.24026517
CCAGGCG8750.037.02538735
AGTCACG6900.036.8436228
CCCAGGC8850.036.6059934
CAGGCGG8950.036.19836
GGCGGTC8850.035.8443738
ACGTCCG7250.035.6855832
GTCTCCC9000.035.2558742
AGGCGGT9050.035.05223537
CGTCCGC7350.034.8939833
GCACACG8450.034.61143511
GCGGTCT9300.034.59768339
CATCTCG7650.034.41749241
CCTGACC7700.033.8969939
ACACGTC8650.033.81117213
CTGACCC7600.033.75372740
GTCACGT7600.033.7461529
CACACGT8700.033.61685612