FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l04n01 ah8335a-062415.3410000000e296.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l04n01 ah8335a-062415.3410000000e296.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2104410
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG1448236.88188138243023TruSeq Adapter, Index 19 (97% over 40bp)
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC28440.13514476741699574No Hit
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA28130.1336716704444476No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA166550.044.4663541
GCACACG165350.044.3002411
TCGGAAG167300.044.2276423
GAGCACA166600.044.0488789
CACGTCT165650.044.04345314
AGCACAC167000.043.92989710
ACACGTC166150.043.92445813
CACACGT167000.043.8625412
ACGTCTG166600.043.82035415
AGAGCAC168200.043.7636158
CGTCTGA166750.043.7404616
ATCGGAA169750.043.6688272
CGGAAGA169600.043.641124
GAACTCC166600.043.5014321
TCCAGTC166050.043.4954325
AGTCACG166800.043.4171328
TCGTATG164700.043.40397645
CCAGTCA166900.043.31435826
GTCTGAA168950.043.3040517
GTCACGT166900.043.2832829
Produced by FastQC (version 0.11.2)