FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l04n01 ah8225b-062415.3410000000e253.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l04n01 ah8225b-062415.3410000000e253.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4313184
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA75360717.472173688857236No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC57902613.424560603025515No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG3402287.888093807266279TruSeq Adapter, Index 15 (97% over 40bp)
TCTTCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC1419393.290817178214516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCGT609550.044.7057761
TCCAGGG801100.044.5771374
CCAGGGA802650.044.53315
AGGGATT805450.044.4709747
CTTCCAG803150.044.4567222
CAGGGAT806150.044.412316
ACTTCCA804450.044.3821641
TTCCAGG804550.044.37483
GCACACG390700.044.35013611
GGGATTT808600.044.3088658
TCGGAAG398100.044.2948233
ATCGGAA399000.044.2559052
ACACGTC392350.044.2553713
CACACGT392300.044.2495412
GAGCACA394600.044.2264069
CGGAAGA398500.044.2164884
TCGTTGG617800.044.212644
ATAAGCC810700.044.19912715
AGCACAC393550.044.19474410
CACGTCT392650.044.1694814
Produced by FastQC (version 0.11.2)