FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l04n01 ah8225a-062415.3410000000e26f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l04n01 ah8225a-062415.3410000000e26f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6039785
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC2212453.663127081510352TruSeq Adapter, Index 4 (100% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA75650.12525280287294993No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC72000.11920954139923855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG242100.043.5456211
ACACGTC243400.043.3407713
ACGTCTG242900.043.33844415
CACGTCT243150.043.2924514
CACACGT243850.043.27924312
GATCGGA246000.043.1735231
CGTCTGA243800.043.1688716
AGCACAC246350.042.885710
TCGGAAG248650.042.707223
GAGCACA248000.042.6928569
TCCAGTC244300.042.68876625
CAGTCAC244650.042.59869427
GAACTCC246000.042.5865421
GTCTGAA247400.042.5770817
CCAGTCA245150.042.51321826
GTATGCC240400.042.50059545
AGAGCAC249800.042.4569248
ACTGACC244850.042.4133632
CTCGTAT241350.042.3239842
AGTCACT246950.042.2348928