FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l04n01 ah8148b-062415.3410000000e239.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l04n01 ah8148b-062415.3410000000e239.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5357503
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG98756318.433270126026994TruSeq Adapter, Index 13 (97% over 40bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA267010.49838516189351645No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC222560.4154174062058388No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1104400.044.716611
TCGGAAG1108100.044.439033
ATCGGAA1112600.044.3830072
GCACACG1098750.044.30320711
GAGCACA1106100.044.258619
CGGAAGA1112550.044.2269024
CACACGT1100850.044.18599712
AGCACAC1105600.044.17930210
ACACGTC1100700.044.1552313
AGAGCAC1111150.044.143748
CACGTCT1099050.044.1326714
ACGTCTG1099150.044.117215
CGTCTGA1103200.043.9735916
GTCTGAA1106700.043.8995817
TCGTATG1091400.043.8404145
GGAAGAG1126300.043.782865
TCTGAAC1107850.043.7524718
GAAGAGC1125350.043.737856
GAACTCC1105500.043.69244821
TGAACTC1111350.043.62847520
Produced by FastQC (version 0.11.2)