FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l04n01 ah8148a-062415.3410000000e246.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l04n01 ah8148a-062415.3410000000e246.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2921193
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG103831635.54424510807742TruSeq Adapter, Index 14 (97% over 44bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA29440.10078074266233009No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1155450.044.927611
ATCGGAA1162700.044.66262
TCGGAAG1160950.044.6562843
CGGAAGA1162100.044.565634
AGAGCAC1157400.044.5615358
GAGCACA1155750.044.552719
GCACACG1152500.044.53388611
AGCACAC1155650.044.50984210
GAAGAGC1161800.044.4741026
CACACGT1154400.044.45322412
ACACGTC1154750.044.4475413
GGAAGAG1165450.044.423615
AAGAGCA1165400.044.344447
ACGTCTG1155550.044.33770415
CACGTCT1155900.044.3293614
CGTCTGA1157700.044.2713416
GTCTGAA1158750.044.2409317
TCTGAAC1158650.044.19620518
TCGTATG1124400.044.19161245
GAACTCC1156750.044.11309421
Produced by FastQC (version 0.11.2)