FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l04n01 ah3337b-062415.3410000000e22c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l04n01 ah3337b-062415.3410000000e22c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1683929
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC63818037.89827243310139TruSeq Adapter, Index 5 (100% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA195561.1613316238392475No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC149970.8905957436447737No Hit
TCTTCAGTTTAAAGATTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC35440.21046017973441875No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATTTCGTATGCC18810.11170304686242709TruSeq Adapter, Index 5 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA700850.044.9452631
ATCGGAA703950.044.7476842
TCGGAAG702850.044.744893
CGGAAGA703050.044.7065544
GAGCACA699600.044.6517759
AGAGCAC701050.044.6236088
AGCACAC699300.044.60260410
GCACACG697650.044.59845411
GAAGAGC704200.044.5696456
CACACGT697350.044.56925212
ACACGTC696450.044.55577513
GGAAGAG706500.044.526465
AAGAGCA704700.044.5106127
CACGTCT695700.044.48607314
ACGTCTG697300.044.42725815
CGTCTGA697800.044.37930316
GTCTGAA699250.044.329117
TCTGAAC698500.044.299418
GTATGCC693300.044.2028545
CTGAACT700400.044.17090219
Produced by FastQC (version 0.11.2)