FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l04n01 ah3337a-062415.3410000000e21f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l04n01 ah3337a-062415.3410000000e21f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3430776
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG70450020.53471284630649TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGGTCTCGTATG44390.12938763708268916TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATT40190.11714550877119345TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGGATG36570.1065939600836662TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA840200.044.74021
TCGGAAG841850.044.6030583
GCACACG832000.044.59905611
GAGCACA836100.044.5983169
AGAGCAC838450.044.5799948
AGCACAC834900.044.57348610
ACACGTC833900.044.50013713
CGGAAGA844000.044.4867674
CACACGT834450.044.47889312
ATCGGAA845750.044.458562
CACGTCT833100.044.44565214
ACGTCTG833550.044.3980115
CGTCTGA836250.044.2728516
GAAGAGC847050.044.230976
GTCTGAA839050.044.1733717
GGAAGAG849750.044.153975
TCTGAAC839800.044.09641318
AAGAGCA849300.044.0925947
CTGAACT841300.043.98901719
GAACTCC838000.043.98288321
Produced by FastQC (version 0.11.2)