FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l03n01 bt5 -chx #8.3410000000dd0a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l03n01 bt5 -chx #8.3410000000dd0a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6953924
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG322020.4630766744071405No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG261030.37537079784018346No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA212000.30486384378086384No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG183220.26347713895061264No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178130.256157530625874No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC134830.1938905285706315No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC130990.18836846649460073No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG102810.1478445838637293No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC97140.13969091407959017TruSeq Adapter, Index 8 (100% over 51bp)
GCTTAACGTAATTCAACAGAAATTATATGATAATCATCGCAAGACCGGCAA91920.1321843609449859No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTT88950.12791339105805585No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT86900.12496541521017485No Hit
GCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGCG81990.11790465354525013No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA79830.11479849362748284No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGA75440.1084855112020206No Hit
CGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTGCAGGGAGGAGTCCTGG74120.10658730236338505No Hit
GCGAGATCAAGATGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCCG69870.1004756451177781No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATG69730.10027431993792281No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG15600.033.74786411
CGTATGC12900.032.96658744
ACGTCTG16450.031.32043315
CACACGT16800.031.20338212
ACACGTC16850.030.84330213
GTATGCC14450.030.36511245
CTCGTAT15900.028.30457542
AGCACAC19000.028.06401410
CACGTCT18750.027.8382414
TCGTATG15900.026.88837843
CGTCTGA19500.026.53773716
GTCACAC19500.026.30659729
GAGCACA21650.024.9406859
CAGTCAC22050.023.77417827
AGAGCAC22800.023.0906438
TCTCGTA19650.022.78842441
TCACACT23150.022.74170530
CCAGTCA23650.022.45101226
TCGGAAG23550.022.1638853
CTGAACT24000.022.03112419
Produced by FastQC (version 0.11.2)