FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l03n01 bt5 -chx #7.3410000000dcfc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l03n01 bt5 -chx #7.3410000000dcfc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8695374
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG405060.4658339020265258No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG312230.35907598684081904No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA256650.2951569420705768No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG210310.2418642372369492No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC164260.1889050430723279No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC160960.18510992166639412TruSeq Adapter, Index 7 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133890.1539784257698404No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG130840.15047081356132583No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC129390.14880326021629434No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA123600.14214454720406505No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT117270.13486481432541025No Hit
GCTTAACGTAATTCAACAGAAATTATATGATAATCATCGCAAGACCGGCAA109450.1258715266301369No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGA106140.12206490485630636No Hit
GCGAGATCAAGATGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCCG104270.11991433605961055No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAG99140.11401464732856804No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG97120.1116915730134207No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTT93280.10727543174106141No Hit
GCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCATGG92800.10672341408201648No Hit
GCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGCG90450.10402082762627576No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGC89070.10243377685652164No Hit
GCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAGG88400.1016632522074381No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC20600.033.86040544
ACGTCTG23000.033.845715
GCACACG23100.033.60178411
GTATGCC22000.032.0124845
CACACGT24450.031.65445112
ACACGTC25200.030.80110413
CTCGTAT24500.029.6665242
CACGTCT26550.029.23511314
TCGTATG24300.028.89105643
AGCACAC27000.028.74819410
CGTCTGA27900.027.98243316
AGTCACC29250.026.3832628
CAGTCAC30200.025.9258227
CATCTCG29600.025.46819739
GAGCACA31550.025.0301239
CTGAACT31500.024.71344219
TCTCGTA30150.024.33102641
GCTGACT42900.023.4127541
TCGGAAG33600.023.167923
CCAGTCA33600.022.96730426
Produced by FastQC (version 0.11.2)