FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l03n01 bt5 -chx #6.3410000000dcef.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l03n01 bt5 -chx #6.3410000000dcef.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8385506
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG435630.5195035338356445No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG354040.4222046946242719No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA303430.3618505549933421No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG192650.22974165184545808No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC176180.21010061885353132No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153400.18293469708327678No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC152600.1819806699798438No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG142270.16966179500676526No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT123240.14696787528385288No Hit
GCTTAACGTAATTCAACAGAAATTATATGATAATCATCGCAAGACCGGCAA121390.14476168760716407No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC119650.1426866786571973TruSeq Adapter, Index 6 (100% over 51bp)
GCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGCG111750.13326566101079648No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGA109980.131154876044451No Hit
GCGAGATCAAGATGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCCG108800.1297476860668873No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAG105400.1256930708772971No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG103490.1234153311678508No Hit
CCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGC99440.11858556895672127No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGC93030.1109414267904644No Hit
GCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCATGG92450.1102497571404755No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCC92230.1099873996870314No Hit
GCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAGGATGTCCC91580.10921225266549209No Hit
GCTCGGTGGAGGCCTCCCAGCCCATGGTCTTCTTCTGCATTACGGGGCCGT91140.10868753775860394No Hit
GTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATC90240.10761425726724183No Hit
CCGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGTTTGAACGA89780.10706569168276786No Hit
CGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTGCAGGGAGGAGTCCTGG88750.1058373817870979No Hit
GCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAGG87830.10474025061814993No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA86130.1027129430233548No Hit
CCGGCGCCTACAAGACCGACATCAAGCTGGACATCACCTCCCACAACGAGG85560.10203319871215882No Hit
GTCTTCTTCTGCATTACGGGGCCGTCGGAGGGGAAGTTGGTGCCGCGCAGC84400.10064985941218096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG19800.035.33905811
ACACGTC20750.033.612513
TCACGCC21400.032.5921430
ACGTCTG21600.032.49810815
CACACGT21600.032.28997812
CACGCCA20500.031.71860731
AGTCACG22250.031.24667228
CGTATGC17300.031.08404244
CTCGTAT18600.030.00218642
CACGTCT23350.029.96613314
GTATGCC18050.029.91800745
AGCACAC23600.029.3628710
CGCCAAT20600.029.27118533
GTCACGC23600.029.26858129
TATCTCG20000.028.35274339
ACGCCAA23200.028.02721832
CGTCTGA25450.027.75886216
TCGTATG19950.027.51998343
CAGTCAC26400.026.50492727
GCCAATA23750.025.38890334
Produced by FastQC (version 0.11.2)