FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l03n01 bt5 -chx #2.3410000000dcac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l03n01 bt5 -chx #2.3410000000dcac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8392852
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG407200.48517476538368604No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG346350.4126725932972487No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA281370.33524956713164966No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC212580.25328696371626713TruSeq Adapter, Index 2 (100% over 51bp)
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG173840.2071286375596758No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC170900.2036256566897641No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC136260.1623524399095802No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG125500.14953200652174017No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA125080.14903158068318134No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124900.14881711246665616No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT110010.13107582499965448No Hit
GCTTAACGTAATTCAACAGAAATTATATGATAATCATCGCAAGACCGGCAA109380.13032518624181624No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTT106720.12715582259761044No Hit
GCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGCG105230.12538050236081846No Hit
GCGAGATCAAGATGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCCG105190.12533284275714618No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGA100460.11969709462290054No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG97300.11593198593279137No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAG93780.11173794080963181No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCC89780.10697198044240504No Hit
GCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAGGATGTCCC89490.1066264483157811No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA85740.10215836047150599No Hit
CCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGC84290.10043069983838629No Hit
CGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTGCAGGGAGGAGTCCTGG84070.10016857201818881No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGC84050.10014474221635267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG27400.037.278311
CACACGT27750.036.64597312
CGTATGC26650.036.0521144
GTATGCC27200.035.65443445
ACGTCTG28750.035.606115
ACACGTC28900.035.49892413
CTCGTAT27650.034.99632642
CACGTCT31300.032.8490414
CGTCTGA31400.032.6733616
CGATGTA30850.032.67281734
CCGATGT31350.032.3666433
AGCACAC32000.032.20077510
TCGTATG30150.032.1677743
TATCTCG30900.032.11715339
AGTCACC33450.030.6033328
CAGTCAC33800.030.35281627
TCGGAAG34800.029.4806083
GAGCACA35650.029.2825789
GTCACCG35350.028.89515729
ACCGATG35400.028.66350432
Produced by FastQC (version 0.11.2)