FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l03n01 bt5 -chx #12.3410000000dd4e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l03n01 bt5 -chx #12.3410000000dd4e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7615052
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG402390.5284139885059222No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG304150.3994063336665331No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA251290.3299911806248992No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC187780.2465905682587591TruSeq Adapter, Index 12 (100% over 51bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178120.23390516571653092No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC161160.2116334858908383No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG160210.21038595665531898No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC122290.1605898423280629No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG113930.1496115850554927No Hit
GCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGCG98730.12965111728718334No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT96510.12673583844207498No Hit
GCTTAACGTAATTCAACAGAAATTATATGATAATCATCGCAAGACCGGCAA92730.12177198527337699No Hit
GCGAGATCAAGATGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCCG88190.11581010871626353No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGA86130.11310494005819002No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAG85770.11263219213736163No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTT85210.11189680648273971No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG84610.11110889328135908No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGC82250.10800976802259525No Hit
CGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTGCAGGGAGGAGTCCTGG80560.10579047917203979No Hit
GCTCGGTGGAGGCCTCCCAGCCCATGGTCTTCTTCTGCATTACGGGGCCGT80270.10540965445803917No Hit
CCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGC78310.10283580466686242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC23200.037.823644
GCACACG27850.037.72675311
GTATGCC24300.036.1116645
CACACGT29450.035.6006912
ACGTCTG29150.035.34984615
ACACGTC29400.035.20184313
CTCGTAT26900.034.12870842
CGTCTGA31300.033.2104916
AGCACAC31650.033.12607210
TCGTATG26750.032.88878343
AGTCACC32100.032.03256628
CACGTCT32600.031.74686414
CAGTCAC32750.031.46447427
AGAGCAC34300.030.566778
GAGCACA35500.029.5335259
TCGGAAG35850.028.993973
TCTCGTA32400.028.26580241
CTGAACT36950.028.13248419
ATCTCGT33400.028.09358840
TCCAGTC37150.027.79823925