FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l03n01 bt5 -chx #10.3410000000dd24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l03n01 bt5 -chx #10.3410000000dd24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5593765
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG262010.4683965093277962No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG200270.3580236209422455No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA165180.2952930629012838No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC124870.22323068630877413TruSeq Adapter, Index 10 (100% over 51bp)
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG117900.21077038452634317No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC103850.18565313344411144No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG91920.1643258163330065No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT76510.13677728685420285No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC75780.13547226242074883No Hit
GCTTAACGTAATTCAACAGAAATTATATGATAATCATCGCAAGACCGGCAA73260.13096724656827735No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG70180.12546111608192334No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTT68880.12313709996755316No Hit
GCGAGATCAAGATGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCCG67480.1206343133828468No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGC66270.11847119069177915No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAG65850.11772035471636724No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65430.11696951874095532No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGA64280.11491365833208939No Hit
GCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGCG59780.10686898716696178No Hit
CCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGC59480.10633267575595327No Hit
GCTCGGTGGAGGCCTCCCAGCCCATGGTCTTCTTCTGCATTACGGGGCCGT59470.10631479870891966No Hit
GTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGA58450.10449133991149073No Hit
GTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATC58250.1041337989708184No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCC58130.10391927440641499No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG16650.037.15998511
CGTATGC15900.036.6510944
ACGTCTG17150.036.07660315
CACACGT17900.034.9420912
ACACGTC18400.033.62574813
GTATGCC17550.033.3340645
TCGTATG17650.033.14668343
TATCTCG18400.032.53079639
CACTAGC19250.031.90807731
CACGTCT19550.031.53267714
CGTCTGA19650.031.25826816
ACTAGCT19600.031.223532
AGCACAC20000.031.0481810
CTCGTAT18700.031.0456842
TAGCTTA19850.030.943634
TCACTAG20250.030.44320530
GTCACTA20350.030.29414729
GAGCACA22100.027.9960989
CAGTCAC22200.027.46534227
TCTCGTA21450.027.1703741
Produced by FastQC (version 0.11.2)