FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l02n01 n_60_3.3410000000d935.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l02n01 n_60_3.3410000000d935.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15191772
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC160270.1054978971511684TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG34600.032.25367428
ACGTCTG40150.028.74643315
CGTCTGA40800.027.90246416
CACGTCT43000.026.84097114
GTCACGA39500.025.51812729
CTCGTAT31300.025.44994742
TCGTATG31450.024.89926543
CGTATGC31900.023.98354344
CACACGT48950.023.8081912
ACACGTC49350.023.52403813
ATCGGAA50550.022.7432942
TCTCGTA36850.021.73868641
CAGTCAC53350.021.46621927
GATCGGA53900.021.3104531
GTATGCC37400.020.87847145
TCACGAT49750.020.80304530
TCGGAAG55750.020.783373
GTCTGAA59250.019.6315417
GCACACG62050.018.99960311
CCAGTCA63000.018.53539526
Produced by FastQC (version 0.11.2)