FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l02n01 n_60_2.3410000000d90e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l02n01 n_60_2.3410000000d90e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13417530
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA20700.021.52050216
ACGTCTG21450.020.87292715
CACGTCT24150.018.53923814
CACACGT26250.017.57035412
ACACGTC29250.016.30669413
CTCGTAT19650.016.14663742
TCGTATG20150.015.52257143
ATCGGAA29400.014.9227782
CGTATGC20850.014.8933444
GTCACAC29650.014.87316829
AGTCACA32600.013.80340228
AATCTCG30700.013.26559939
TCGGAAG34050.013.0170183
CAGTCAC34700.012.83835827
GATCGGA35400.012.5964621
TCTCGTA27550.012.3331641
CGCGTAT3501.0913936E-1112.22565651
CACACTT39000.011.65317631
GCACACG41950.011.53100811
GTATGCC26500.011.37878345
Produced by FastQC (version 0.11.2)