FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l02n01 n_60_1.3410000000d8c9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l02n01 n_60_1.3410000000d8c9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11684155
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA22800.024.17609816
ACGTCTG22850.023.92627115
CTCGTAT20400.021.73000542
CACGTCT25550.021.3978614
TCGTATG20700.020.65413543
CGTATGC21000.019.82305544
CACACGT28600.019.74525312
ACACGTC30300.018.3404213
ATCGGAA30250.018.2964382
TCTCGTA26500.017.49215141
GTATGCC25500.016.85533545
AGTCACC34050.016.0568728
TCGGAAG34800.015.96888353
GATCGGA35450.015.9455721
CAGTCAC33550.015.82672927
GCACACG39250.014.73154911
GTCTGAA39150.014.30943717
CCAGTCA41750.013.31104726
TCCAGTC43050.012.90914225
CGGAAGA45300.012.8638094
Produced by FastQC (version 0.11.2)