FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l02n01 n_120_3.3410000000d9bb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l02n01 n_120_3.3410000000d9bb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10110822
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG17050.019.79302815
CGTCTGA17200.019.09720616
CACGTCT18400.018.46309514
CACACGT19400.017.62735712
CGTATGC14050.016.81741744
CTCGTAT14800.016.5737142
ACACGTC21850.016.062713
TCGTATG14650.015.9752843
ATCGGAA23350.014.1637422
AGTCACC25400.013.464148528
TCGGAAG25050.013.3822213
GTAATCT21850.012.97557937
GATCGGA27250.012.397691
TCTCGTA20600.012.34404741
GTCTGAA28200.012.12662317
CAGTCAC28600.012.03622127
AATCTCG23300.011.78179839
TGTAATC25050.011.58652336
GCACACG30900.011.57673711
GTATGCC20400.011.36252645
Produced by FastQC (version 0.11.2)