FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l02n01 n_120_2.3410000000d985.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l02n01 n_120_2.3410000000d985.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10519912
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG15600.023.36478628
CGTCTGA17550.021.28049716
ACGTCTG18000.021.12345715
CACGTCT21150.017.9774114
CACACGT22600.017.42129312
ACACGTC23950.016.25142113
GTCACGG23200.015.90469129
ACGGCTA18300.015.85964832
ATCGGAA24300.015.5545662
GATCGGA25750.014.9567221
TCGTATG13850.014.9478143
CGTATGC14150.014.63075644
CTCGTAT14200.014.42090742
TCGGAAG27550.013.7196363
GTCTGAA30350.012.972692517
CAGTCAC31650.012.01391127
CACGGCT25800.011.94691531
CGGAAGA34600.011.8997024
GCACACG34600.011.83456311
CGCGTAT3451.2951205E-911.0980671
Produced by FastQC (version 0.11.2)