FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l02n01 n_120_1.3410000000d96b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l02n01 n_120_1.3410000000d96b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13044126
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG22050.022.1414315
TATCTCG20900.021.96224439
CGTCTGA23650.020.45330816
CACGTCT24300.020.1838114
CGTATGC22150.020.11550344
CTCGTAT22400.019.99153742
TCGTATG23100.019.38573543
GTCACTA27650.017.57612429
TAGCTTA27200.017.53590434
CACACGT29000.017.37812412
AGTCACT27950.017.22647928
TCACTAG30300.016.11282330
TCTCGTA27800.016.02709641
ATCGGAA30650.016.0023562
GTATGCC29350.015.7947845
ACACGTC32100.015.69986313
GCTTATC31200.015.3636
CACTAGC33350.014.70669831
TTATCTC34150.014.62710838
GATCGGA35200.014.0119111
Produced by FastQC (version 0.11.2)