FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l02n01 k_60_3.3410000000d7a1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l02n01 k_60_3.3410000000d7a1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11451908
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154620.13501680244025713No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGG18600.025.28091832
CACTTAG18950.024.57631731
ACGTCTG20800.023.36383815
CGTCTGA21400.022.28824416
CACGTCT22650.021.65419814
CTCGTAT18950.020.78143542
TCGTATG20350.019.24117343
CGTATGC19450.019.09005744
CTTAGGC24400.019.0873533
TTAGGCA25150.018.96544534
CACACGT26950.018.28264812
ACACGTC27150.018.1479713
TCACTTA26450.017.7780430
AGTCACT26850.017.68101128
ATCGGAA27500.017.1806072
TCTCGTA23700.016.90093641
TAGGCAT29900.016.32870735
GTCACTT29550.016.21776829
TCGGAAG29750.016.0324823
GTATGCC24950.015.33346245
Produced by FastQC (version 0.11.2)