FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l02n01 k_60_2.3410000000d72a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l02n01 k_60_2.3410000000d72a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12960506
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG20100.019.58796315
CTCGTAT17250.019.30628242
TCGTATG18350.018.27158743
CGTCTGA21450.018.04049516
CACGTCT22250.017.59407814
TATCTCG20850.017.48241839
CACACGT22950.017.1554712
CGTATGC21600.015.730544
ACACGTC25600.015.5553813
GTATGCC26300.013.60486545
AGTCACC27850.013.57234428
ATCGGAA28150.013.4271312
TCTCGTA26350.013.06557341
GTATCTC30750.012.36613838
GATCGGA31700.012.149271
CGATGTA31250.012.09562634
CCGATGT31050.012.02861333
TCGGAAG33000.011.6583263
ACCGATG34400.011.11854232
CAGTCAC34250.011.10171827
Produced by FastQC (version 0.11.2)