FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l02n01 k_120_3.3410000000d886.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l02n01 k_120_3.3410000000d886.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9243157
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG13050.024.48211928
ACGTCTG16050.020.74599815
CACGTCT16500.020.58925414
CGTCTGA16800.019.95375416
CACACGT18250.018.12183412
ACACGTC19250.017.29731213
ATCGGAA19400.017.0477852
CTCGTAT11350.015.4646642
GTCACGC20250.015.33284829
TCGGAAG22900.014.63872053
TATCTCG13050.014.48292739
GATCGGA24100.014.1109571
TCGTATG12250.013.40998743
TCACGCC23400.013.07643930
CAGTCAC26700.012.72438527
CGCCAAT17500.012.59939733
CGTATGC12950.012.51142344
GTCTGAA29200.011.86548517
GCACACG30900.011.4311211
AGCACAC32050.011.02137310
Produced by FastQC (version 0.11.2)