FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l02n01 k_120_2.3410000000d843.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l02n01 k_120_2.3410000000d843.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8681642
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115160.13264771802384848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC12350.021.31713344
CTCGTAT12750.021.1780542
TCGTATG12950.020.67697743
ACGTCTG13800.019.72700315
CGTCTGA14800.019.00216716
CACGTCT15600.017.88347414
CACACGT16900.016.24156812
ATCGGAA17450.015.7299292
ACACGTC17850.015.50321413
GTATGCC18400.014.91984845
TCTCGTA18600.014.51712641
GTCACAC19350.014.069629
TCGGAAG20400.013.8964133
GATCGGA20500.013.731371
ACACAGT23400.012.4032732
AGTCACA22500.012.29985328
CAGTCAC22450.012.12680427
GTCTGAA24700.011.93243817
CACAGTG26050.011.22795133
CGGAAGA28100.010.5690264
Produced by FastQC (version 0.11.2)