FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l02n01 k_120_1.3410000000d80f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l02n01 k_120_1.3410000000d80f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11517082
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG18800.018.90854315
CGTCTGA18700.018.52840216
CACGTCT20200.016.92969514
CACACGT20400.016.6534312
CTCGTAT14850.016.0622442
AGTCACT22150.015.23665328
TCGTATG14800.014.59608143
ACACGTC25100.014.34178513
ATCGGAA24000.013.7804992
CGTATGC15550.013.16848644
ACTGACC25350.012.9579332
TCGGAAG27450.012.6222633
AATCTCG24750.012.27280539
GCGGGAT20000.012.1610581
GATCGGA29500.011.9091351
TCTCGTA22600.011.44998241
CAGTCAC30250.011.30541427
GTCTGAA31600.011.24933817
GCACACG33450.011.16531111
GACCAAT31050.010.86897335
Produced by FastQC (version 0.11.2)