FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l01n01 nlp7_chip_input_5_pchx_pn.3410000000d8f0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l01n01 nlp7_chip_input_5_pchx_pn.3410000000d8f0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13142544
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGCC154650.11767128190706456TruSeq Adapter, Index 20 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG68100.037.33117311
ACGTCTG70050.036.1313915
GTCACGT49700.035.6713929
CACACGT71650.035.45014612
ACACGTC71850.035.3514713
CGTCTGA71850.035.25753416
TCACGTG51350.033.1663330
GAGCACA78500.032.4427079
CACGTGG52200.032.36766431
AGCACAC80150.031.97131310
TCGGAAG81700.031.8417363
AGTCACG69500.031.2715328
GTATGCC29250.030.62289445
CTGAACT83200.030.47561319
CGTATGC29300.029.95558244
ACTCCAG84950.029.26615323
AGAGCAC88650.028.7027958
ATCGGAA95200.027.3972762
TCTGAAC94300.026.93575718
GTCTGAA94100.026.84914217
Produced by FastQC (version 0.11.2)