FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l01n01 nlp7_chip_input_3hrs_pchx_pn.3410000000d992.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l01n01 nlp7_chip_input_3hrs_pchx_pn.3410000000d992.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9064272
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG21600.032.08032211
ACGTCTG23800.029.2094415
CACACGT24900.028.37082912
ACACGTC24950.027.86311313
CGTCTGA25750.026.91009316
AGTCACG24300.023.79537628
GAGCACA29700.023.7860379
TCGGAAG30200.023.77183
AGCACAC29750.023.74566810
TCACGAG21900.023.21712530
GTCACGA23250.022.06317529
AGAGCAC34200.020.7220828
ACTCCAG32900.020.65303623
CTGAACT33550.020.58719419
CGTATGC11550.020.06814444
GTATGCC12300.019.57651745
CGAGTGG21700.018.35081133
ACGAGTG21850.018.01890432
ATCGGAA41700.017.4855822
CGGAAGA41650.017.3957234