FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l01n01 nlp7_chip_input_30_pchx_pn.3410000000d95f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l01n01 nlp7_chip_input_30_pchx_pn.3410000000d95f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9712572
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGCC143270.1475098460016564TruSeq Adapter, Index 22 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG25500.035.90771533
GCACACG32250.034.74008611
ACGTCTG33250.033.4922915
CGTACGA27400.033.17160834
CACACGT35000.032.07478712
ACCGTAC28850.031.9721332
ACACGTC36300.030.92610413
GTACGAT29350.030.73758935
CGTCTGA36250.030.72051616
GTCACCG31900.030.39735229
CACCGTA30800.030.38618531
CGTATGC22850.028.95878244
GTATGCC23400.028.37489345
GAGCACA40300.028.1357849
AGTCACC35050.028.11583128
AGCACAC40850.027.7017510
TCACCGT34500.027.58412730
TCGGAAG41500.027.1683983
CTGAACT44350.025.46499619
TACGATC36900.024.75457836
Produced by FastQC (version 0.11.2)