FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l01n01 nlp7_chip_input_10_pchx_pn.3410000000d928.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l01n01 nlp7_chip_input_10_pchx_pn.3410000000d928.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12984387
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG66200.037.0094611
ACGTCTG67300.035.96996715
CACACGT69450.035.2127712
ACACGTC70750.034.3431513
CGTCTGA70600.034.2249216
GTCACGT42050.033.22641429
GAGCACA76800.031.9305559
AGTCACG66250.031.4140428
AGCACAC78750.031.05430610
TCGGAAG81500.030.4567033
CTGAACT82900.029.22833319
AGAGCAC86400.028.5129138
ACTCCAG84600.028.29703123
GTATGCC24400.027.85439945
CGTATGC24250.027.84076544
CGTTTCG30150.026.86332333
TCTGAAC91800.026.6641318
ATCGGAA96150.025.8394372
CGGAAGA97250.025.7049924
CAGTCAC91700.025.54167227