FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l01n01 nlp7_chip_input_0_pchx_pn.3410000000d8bc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l01n01 nlp7_chip_input_0_pchx_pn.3410000000d8bc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12959333
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG72400.036.4811615
GCACACG72750.036.46026611
CGTCTGA75500.035.0428516
GTCACGT52850.034.86529
ACACGTC76150.034.77327713
CACACGT77000.034.50628712
TCACGTG51700.033.3332230
AGTCACG75050.032.1068728
GAGCACA83400.031.9933249
AGCACAC84150.031.65458510
CACGTGA38700.030.86900331
TCGGAAG88100.030.6022973
CTGAACT88350.030.0737419
ACTCCAG86650.029.8602523
AGAGCAC93550.028.6904538
GTATGCC29150.028.10343745
CGTATGC28750.028.02399444
CGTGAAA44250.026.53971733
ATCGGAA102350.026.4513342
TCTGAAC101100.026.3921218