FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l01n01 nlp7_chip_5_pchx_pn.3410000000d6f2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l01n01 nlp7_chip_5_pchx_pn.3410000000d6f2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15634287
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCC13732098.783317077395344TruSeq Adapter, Index 14 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATTCC342950.21935762084961086TruSeq Adapter, Index 14 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCCTATGCC204610.1308726135064554TruSeq Adapter, Index 14 (97% over 44bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA160060.10237755006032574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG1953900.044.2232111
ACGTCTG1951250.044.12069715
CACACGT1960200.044.07763312
CGTCTGA1951600.044.07589716
ACACGTC1959200.044.05764413
AGCACAC1966850.043.99266410
GAGCACA1971550.043.932299
CTGAACT1961100.043.73330719
AGAGCAC1990850.043.5900238
CACGTCT1978850.043.57012614
GTCTGAA1974650.043.52607717
TCTGAAC1973200.043.51957718
TCGGAAG2005500.043.4763573
CACAGTT1936400.043.47148531
ACTCCAG1963350.043.4529323
GTATGCC1498700.043.4408645
ACAGTTC1935050.043.42624332
GTCACAG1947300.043.41786629
CTCCAGT1966050.043.29941624
CGGAAGA2011700.043.2918244
Produced by FastQC (version 0.11.2)