FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l01n01 nlp7_chip_3hrs_pchx_pn.3410000000d836.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l01n01 nlp7_chip_3hrs_pchx_pn.3410000000d836.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10400671
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCC4323384.156827958503831TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAGAGATCTCGTATGCC213420.20519829922511731TruSeq Adapter, Index 2 (97% over 36bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA184080.17698858083290972No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC135660.13043389219791685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG860450.044.0878511
ACGTCTG859150.043.98435215
CACACGT862400.043.969912
ACACGTC861550.043.94799813
CGTCTGA860900.043.8531316
AGCACAC868550.043.7518110
GAGCACA870900.043.6752939
GTATGCC534000.043.570445
TCGGAAG880050.043.4927753
CTGAACT867450.043.3741719
AGAGCAC879650.043.3533828
AGTCACG824350.043.3005228
GTCACGT623000.043.27384629
CGGAAGA884350.043.2301374
CACGTCT875550.043.18617214
CGTATGC537000.043.166944
ATCGGAA889250.043.115992
TCTGAAC873550.043.10734618
GTCTGAA876250.043.06694817
ACTCCAG871350.043.04846623
Produced by FastQC (version 0.11.2)