FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l01n01 nlp7_chip_30_pchx_pn.3410000000d7e4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l01n01 nlp7_chip_30_pchx_pn.3410000000d7e4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16072927
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCC12913778.03448556694123TruSeq Adapter, Index 16 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCCTATGCC935620.5821092822732288TruSeq Adapter, Index 16 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCTCTCGTATGCC391190.24338441902958932TruSeq Adapter, Index 16 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATTCC341730.2126121769855609TruSeq Adapter, Index 16 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCTCTCCTATGCC288350.17940105122109992TruSeq Adapter, Index 16 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCCTATTCC287670.17897797955531064TruSeq Adapter, Index 16 (97% over 44bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA287340.1787726653645599No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC222830.13863685189387098No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTCTGCC171320.10658917321033064TruSeq Adapter, Index 16 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCTCTCCTATTCC165790.1031486051047205TruSeq Adapter, Index 16 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGCATGCC163290.1015931945687304TruSeq Adapter, Index 16 (97% over 45bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG3007200.044.4422211
ACGTCTG3006400.044.3956715
CACACGT3012250.044.35725412
CGTCTGA3008050.044.3503816
AGCACAC3019600.044.34390610
ACACGTC3012050.044.3430213
GAGCACA3022350.044.340179
TCGGAAG3050900.044.173213
CTGAACT3015550.044.15306519
AGAGCAC3044050.044.095048
GTATGCC1424150.044.07028645
CGGAAGA3058850.044.0102654
TCTGAAC3027950.044.0079118
CACGTCT3033300.043.99973714
AGTCACC2987700.043.9991128
GTCACCC2988600.043.96875829
CACCCGT2389550.043.9639431
GTCTGAA3035000.043.94692617
ATCGGAA3072600.043.9467932
GATCGGA3072850.043.933341
Produced by FastQC (version 0.11.2)