FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l01n01 nlp7_chip_10_pchx_pn.3410000000d77a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l01n01 nlp7_chip_10_pchx_pn.3410000000d77a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11724514
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGCC153867813.123597276612061TruSeq Adapter, Index 15 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATTCC420790.3588976054785725TruSeq Adapter, Index 15 (97% over 48bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA359100.3062813520458076No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC275560.23502893168962058No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGGC138030.11772769429931168TruSeq Adapter, Index 15 (97% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG1985400.044.44029211
ACGTCTG1989000.044.2750215
GAGCACA1998400.044.2746629
AGCACAC1996600.044.2586110
CGTCTGA1989200.044.21967316
CACACGT1995300.044.21753712
ACACGTC1995150.044.1971813
TCGGAAG2019050.044.117083
AGAGCAC2010700.044.0955738
CGGAAGA2023350.043.979684
CTGAACT1995600.043.9401319
ATCGGAA2031750.043.9343572
CACGTCT2006350.043.9168214
GTATGCC1662650.043.9150945
TCTGAAC2000600.043.88429318
GATCGGA2033850.043.8681641
GTCTGAA2007100.043.79466617
ACTCCAG1995400.043.7289823
AAGAGCA2033400.043.6973537
GAACTCC2005600.043.66607321
Produced by FastQC (version 0.11.2)