FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l01n01 nlp7_chip_0_pchx_pn.3410000000d67b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l01n01 nlp7_chip_0_pchx_pn.3410000000d67b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12437088
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCC8237726.623511870302759TruSeq Adapter, Index 13 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATTCC460670.37040020943809354TruSeq Adapter, Index 13 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGGATGCC261300.21009741187004546TruSeq Adapter, Index 13 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGGC211660.1701845319418822TruSeq Adapter, Index 13 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCCTATGCC129140.10383459536508867TruSeq Adapter, Index 13 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG1548100.043.97772615
GCACACG1556300.043.93972811
CGTCTGA1548850.043.91866716
ACACGTC1554250.043.9180713
CACACGT1556150.043.9121612
GAGCACA1570000.043.666829
AGCACAC1568950.043.6628910
CTGAACT1553550.043.63729519
TCTGAAC1565500.043.36081318
CACGTCT1575000.043.28375614
ACAGTCA1517350.043.28261632
AGAGCAC1587600.043.2805188
GTCTGAA1571900.043.23457317
ACTCCAG1561200.043.1962323
GTCACAG1542600.043.16603529
GTATGCC904400.043.13831745
GAACTCC1570550.043.0603721
CACAGTC1529250.043.05744631
TCGGAAG1606450.043.035673
CAGTCAA1525800.042.98835433
Produced by FastQC (version 0.11.2)