FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l01n01 bzip1_chip_pchx_pn.3410000000d879.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l01n01 bzip1_chip_pchx_pn.3410000000d879.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10917176
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATGCC4368924.001877408589913TruSeq Adapter, Index 18 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCCTATGCC310040.28399285676075936TruSeq Adapter, Index 18 (98% over 51bp)
ACTTCCAGGGATTTATAAGCCGATGACGTCATAACATCCCTGACCCTTTAA245120.2245269289420634No Hit
TATTCGTTGGAATTCCTCGGGGAATTCGGTATTCCCAGGCGGTCTCCCATC188730.1728743770366989No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATTCC145310.13310218686590744TruSeq Adapter, Index 18 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG1402250.044.22707711
ACGTCTG1397800.044.18117515
CACACGT1402300.044.1805812
ACACGTC1401750.044.1497713
AGCACAC1408850.044.12049510
CGTCTGA1400100.044.09895316
GAGCACA1411350.044.0764249
AGAGCAC1415950.044.0253838
TCGGAAG1430200.043.8366853
TCTGAAC1409250.043.7106618
CGGAAGA1435100.043.6286624
GTCACGT1078900.043.59778229
AGTCACG1312350.043.5853928
CTGAACT1412450.043.573819
ACGTCCG849900.043.5396832
TCACGTC1044050.043.50462730
CACGTCT1421400.043.5030214
ATCGGAA1444100.043.496732
GTCTGAA1419800.043.47756617
CACGTCC1043650.043.44780731
Produced by FastQC (version 0.11.2)