FastQCFastQC Report
Wed 5 Aug 2015
C7D4YACXX l01n01 bzip1_chip_input_pchx_pn.3410000000d9c8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D4YACXX l01n01 bzip1_chip_input_pchx_pn.3410000000d9c8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9329631
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCC304840.3267438980169741TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG85100.040.7386511
ACGTCTG86850.039.7623615
CGGTAGC55800.039.55262833
ACACGTC88200.039.35780713
CGTCTGA88500.038.97018416
CACACGT89400.038.8043512
ACGGTAG60800.037.7426632
GAGCACA92400.037.6420639
CACGGTA62550.037.19025431
AGCACAC93450.037.17077310
AGTCACG87850.036.85325628
GTATGCC39800.036.69716645
GGTAGCA59550.036.68411334
AGAGCAC94950.036.6785288
TCGGAAG96200.036.5652733
CGTATGC40050.036.29913344
GTCACGG90700.035.52103429
TCTGAAC97200.035.5062118
GTAGCAT62850.034.75779335
CATCTCG59550.034.57308239
Produced by FastQC (version 0.11.2)