FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l08n01 579.34100000033e20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l08n01 579.34100000033e20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46711980
Sequences flagged as poor quality0
Sequence length51
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG61050.029.1875111
GATCGGA68750.025.9002271
CACACGT69300.025.71280512
ACGTCTG72650.024.58908715
CACAGTC73800.023.991731
TCCAGTC84400.021.16629625
CAGTCAC83050.021.10392427
ACACGTC86750.021.0852413
AGCACAC84000.021.07842310
CACGTCT88750.020.71155214
CCAGTCA89200.019.82551626
GAGCACA90200.019.7792249
GTCACAG93350.019.13587829
CTCCAGT96050.018.8332624
AGAGCAC96050.018.7385188
CTCGTAT96150.018.46225544
ACTCCAG102550.017.68341623
CGTCTGA109150.016.75854916
GAACTCC108250.016.419821
ATCTCGT112750.015.90372542