FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l08n01 543.34100000033e4a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l08n01 543.34100000033e4a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40568851
Sequences flagged as poor quality0
Sequence length51
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG41000.024.85842311
GATCGGA49700.020.5174431
CACACGT49550.020.47821612
ACGTCTG52400.019.49313415
CCGTATC53350.018.97836138
CAGTCAC60400.017.02313627
TCCAGTC62750.016.63672325
ACACGTC61550.016.63192613
CACGTCT61950.016.56085414
AGCACAC62700.016.32643310
GAGCACA63750.016.0928179
AGTTCCG66350.015.86943434
GTCACAG65700.015.85480529
CCAGTCA65400.015.82499526
GTATCTC69400.014.5891140
CTCCAGT71950.014.38435924
ACTCCAG73200.014.23093323
AGAGCAC75600.013.8679288
GTTCCGT76700.013.81646935
CTCGTAT75450.013.5977844
Produced by FastQC (version 0.11.2)