FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l07n01 lbd38_-chx_9.341000000340f2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l07n01 lbd38_-chx_9.341000000340f2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11809466
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG577220.4887773926441721No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG553820.4689627795194127No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA376980.3192184981099061No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC292990.24809758544543842No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG229270.19414086970570898No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT225880.19127029113763483No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT202890.17180285713172805No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG182130.15422373882104407No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTT172210.14582369770148795No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCC170420.14430796447527772No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC169940.14390151087271855No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT165500.14014181504904624No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCC164120.13897326094168863No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGA157730.13356234735761974No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCTT149890.12692360518248666No Hit
GCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGG139790.11837114396197085No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG135030.11434047906992578No Hit
GCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCA134830.11417112340219278No Hit
GCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGCG134770.11412031670187288No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC130600.11058925102964012No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA129970.11005578067628122No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC123060.10420454235610653TruSeq Adapter, Index 9 (100% over 51bp)
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAG122790.103975912204667No Hit
GGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATC122050.10334929623405495No Hit
CGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTG120530.10206219315928426No Hit
GGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTC120180.10176582074075152No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA119420.10112226920336619No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC19350.030.23301944
GCACACG21250.027.84559611
GTATGCC21950.027.2669345
ACGTCTG23000.025.8250615
ACACGTC23650.025.4954913
TCGTATG23500.025.27687543
CTCGTAT23700.025.06356642
CACACGT24550.024.46918312
CACGTCT24850.024.0835514
AGTCACG24950.023.08398228
AGCACAC26800.022.58181410
GTCACGA25850.022.1062229
GCTGACT49850.021.9024121
CGATCAG26800.021.65885233
TCACGAT28700.020.14617330
TCTCGTA29750.020.11718841
CGTCTGA31100.019.3884616
ACTCTAG58750.019.0424775
CAGTCAC30950.018.97251327
CTGACTC57700.018.8431532
Produced by FastQC (version 0.11.2)