FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l07n01 lbd38_-chx_8.341000000340e5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l07n01 lbd38_-chx_8.341000000340e5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11132109
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG322000.2892533660962177No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG283230.25442618285537805No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA182150.16362577836778278No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC176430.1584874887588686TruSeq Adapter, Index 8 (100% over 51bp)
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT136700.12279793523401542No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC125280.11253932206377065No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG116480.10463426112697963No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG113750.10218189563181604No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC26450.033.26182644
GCACACG28000.031.33737211
GTATGCC28900.030.28633745
CACACGT29300.030.02376412
ACACGTC29650.029.97287413
ACGTCTG30550.029.23716715
TCGTATG31700.027.7532943
CACGTCT33100.026.84881414
AGCACAC33400.026.6745410
CTCGTAT33700.026.37328142
GTCACAC34900.025.33467529
CGTCTGA37150.024.2246316
TCTCGTA38800.022.9056641
CAGTCAC41850.021.39615827
CTGAACT42700.021.12830519
GAGCACA42700.020.917559
AGAGCAC44100.020.5085838
TCGGAAG44250.020.2453273
TCACACT46150.019.6953530
CCAGTCA45600.019.68594426
Produced by FastQC (version 0.11.2)