FastQCFastQC Report
Tue 25 Aug 2015
C7D1TACXX l07n01 lbd38_-chx_6.34100000034038.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7D1TACXX l07n01 lbd38_-chx_6.34100000034038.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10779783
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG561370.5207618743345761No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG497380.46140075361442806No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA351730.32628671653223446No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC256850.2382701024686675No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT217190.20147900936410312No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT210390.19517090464622525No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG209200.19406698632059663No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG186790.17327807062535489No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCC158940.14744267115581083No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT158360.1469046269298742No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGA157010.14565228261088373No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTT152910.14184886653098677No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCC151590.14062435208575164No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCTT136210.12635690347384543No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA136170.126319796975505No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG136020.12618064760672826No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC130840.12137535607163892No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA124410.11541048646341026No Hit
GCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGG123330.11440861100821789No Hit
GGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATC121230.11246051984534382No Hit
GCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCA119230.11060519492832091No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC117860.1093342973601602No Hit
CGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTG116770.10832314528038273No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAG115390.10704297108763691No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG16150.027.86206411
CGTATGC16350.027.79942544
ACGTCTG17850.025.3355215
GTATGCC17800.024.90281145
TCACGCC18100.024.85954530
GTCACGC18100.024.85942829
GCTGACT43600.023.3892021
ACACGTC19500.023.30626113
CACGCCA19200.022.96659331
AGTCACG19900.022.83694628
CACACGT20000.022.83609812
TATCTCG20500.022.17091239
TCGTATG20500.021.95221343
CTCGTAT21050.021.48553342
CACGTCT21550.021.29868714
ACTCTAG49100.021.1338585
CGCCAAT20800.020.98428733
AGCACAC22250.020.93052110
CTGACTC48450.020.6740512
ACGCCAA22550.019.754732
Produced by FastQC (version 0.11.2)